Journal: Bio-protocol

Article Title: FlashTag-mediated Labeling for Intraventricular Macrophages in the Embryonic Brain

doi: 10.21769/BioProtoc.5166

Figure Lengend Snippet: A. The CFSE stock solution was prepared by dissolving the CFSE provided in the kit using the attached DMSO. B. The 2.5% CFSE working solution was prepared in a 1.5 mL tube. C. Glass capillaries marked every 3.5 mm to measure out 1 μL of CFSE working solution. D. The CFSE working solution was loaded into the glass capillary using a microloader pipette. E. The glass capillary filled with the solution was attached to the homemade pipette holder. F. After anesthetizing the mother, one side of the uterus was exposed. G. 1 μL of the CFSE working solution was injected into the right lateral ventricle of the embryo located inside the uterus. H. An image taken immediately after the solution was injected into the right lateral ventricle of the embryo. The space in the right lateral ventricle was visualized by the green color of the Fast Green. A small amount of the solution leaked into the left lateral ventricle. I. A magnified image of the fetal head shown in H.

Article Snippet: Eppendorf microloader (Merck, catalog number: EP5242956003) 3.

Techniques: Transferring, Injection

Journal: Journal of visualized experiments : JoVE

Article Title: Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

doi: 10.3791/64439

Figure Lengend Snippet: Materials

Article Snippet: Femtotips Microloader , Fisher scientific , E5242956003 , .

Techniques: Injection, Microscopy, Imaging